Is there any effective FEM solver to perform simulation of balloon inflation with large deformation (e.g. 300% strain)?

Hi! Thank you for your time, attention, and support. I am currently using FEM solver Abaqus to perform a set of balloon inflation simulations, trying to understand the deformation shape, stress,...

14 February 2021 2,524 4 View

Why are we measuring gel content of NBR latex? Does gel content related to the amount of cross-linked in a polymer?

Hi good day to everyone! I am actually studying the gel content of synthetic latex. Refer to ASTM 3616, gel content is measure of insoluble rubbers in the chosen solvent. So, I just would like to...

07 February 2021 605 4 View

How to delete a copy of a paper, and how to delete a co-author's name?

I mistakenly uploaded a copy of the same paper and entered my alternative name. How to delete them out? (I couldn't navigate to the right buttons to do it. So from where to where is what I don't...

07 January 2021 7,694 6 View

What effect will different solvents have on the ATRP reaction?

I want to do an ATRP reaction, synthesize an ATRP initiator, use DMF as a solvent, and mix DMF, BIBB and TEA to turn red. Why does it turn red? Xylene has been used as a solvent before, and there...

04 January 2021 8,034 3 View

Species identification.Who can tell me the scientific names of these Gastropods?

Species identification. I've been doing research on communities of marine macrobenthos recently, but some of them can't be identified, which hinders my research. Who can help me identify these...

14 December 2020 8,357 3 View

Cell shrinkage during ROS assay(H2DCFDA)?

Hi! I'm Yu, studying about health of MSC. These days, I'm doing ROS assay with H2DCFDA which requires 5~30 minutes of staining with PBS or HEPES or HBSS. In our lab, We use PBS w/o Mg/Ca for...

13 December 2020 8,813 9 View

Can I fix the cells after H2DCFDA assay for FACS analysis?

Hi ! I'm Yu, and planning to analyze ROS assay with H2DCFDA(Thermo) on MSCs. A lot of protocols instruct that treat H2DCFDA at specific concentration for 5~30 min, 20~30 min of recovery time...

10 December 2020 973 1 View

How to reconstruct a Lamb wave by a series of pulses superposed?

Lamb wave is useful for solid structure health monitoring, and it consists many modes. Unlike ultrasonic bulk wave, when given a pulse excitation (for example, 5-cycle sinusoidal pulse), only...

08 December 2020 5,440 8 View

How to interpret the significant effect of covariates when conducting a Two-way ANCOVA?

06 December 2020 8,560 4 View

Did anyone do microglia Oil Red O staining?

I need to stain adiposome in microglia, but I don't know how to confirm that are really success? method : Removed media and washed cells with PBS, then added 4 % paraformaldehyde for 20 minutes at...

24 November 2020 2,359 2 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

Why initial concentration of Pb not the same as calculated one when testing using AAS?

I am currently doing research on the adsorption of heavy metal using magnetic reduce graphene oxide. I use AAS to determine the concentration of the heavy metal. I prepare the Pb solution with...

26 February 2021 9,580 4 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

Are there lab markers/pens resistant to Roti-Histol?

Hi, I'm looking for a pen/marker to write on the smooth, transparent (non-frosted) part of microscopy slides which does not get washed away by alcohols and most importantly Roti-Histol. I want to...

24 February 2021 9,902 5 View

RNA degraded after good nanodrop readings?

23 February 2021 8,850 2 View

Is this the write procedure for acid digestion of soil for heavy metal analysis using AAS?

How to do acid digestion of soil sample with 3:1 aquaregia in an open vessel?

23 February 2021 3,053 7 View