How to extract Mannitol from seaweeds?

More Vaibhav A. Mantri's questions See All

Collaboration with researchers from different countries asking for the morphometric data based on herbarium samples?

Dear Colleagues: Good day and greetings from CSMCRI, India! I am thankful to all those who have responded to my previous query posted in the list last year. We are pleased to inform that our work...

19 June 2024 2,468 0 View

What are the main causes of climate change due to heat waves ?

* I want to know, what are the main factors responsible for changing climate ? * What are useful remidies to overcome or manage global warming ?

30 May 2024 10,118 2 View

Current Trends in IoT Automation?

I am currently delving into the latest trends in IoT automation and am keen to gather insights on the most promising developments in this area. Could you share your experiences or recent findings...

24 April 2024 7,509 0 View

Impact of AI Tools on Academic Research?

As AI technology, such as ChatGPT, continues to advance, I'm curious about its impact on academic research. How do researchers view the integration of AI tools in their work? Are these tools seen...

23 April 2024 7,832 4 View

Soil Physics Problem?

A sprinkler adds 15 cm of a bromide solution (i.e.,15 cm3 of water for each 1 cm of area) at a rate of 2 cm h-1. The volumetric water content (Theta) of the soil is 0.30 cm3 cm-3. Assume...

27 January 2024 7,107 1 View

If anyone can solve this problem in soil physics, please let me know?

Imagine you have a field with excessive salt buildup. Assume the volumetric water content is 0.35 cm3 cm-3 and the root zone is 120 cm deep. If the soil in the field is a clay loam soil with Ks=...

20 January 2024 6,449 4 View

Why my plasmid construct is not expressing after cloning?

I have been trying to express my construct for the last couple of weeks but after several attempts, it's still failing although similar parts (five inserts for golden gate assembly) have been used...

12 December 2023 4,729 8 View

How to do proper cloning calculations for my plasmid construction pcr reaction?

Hi, I have recently started my cloning experments and don't have much experience in doing ligation reactions. I have five insert parts with each having 10ng/ul concentration and i need 200ng of...

03 December 2023 1,786 4 View

How to resolve issue in computation of nodal forces in LS-Dyna?

Greetings to All, I am currently conducting a steady-state frequency response analysis of a human body finite element model using LS-Dyna. I have applied an acceleration load and obtained the...

03 November 2023 5,428 1 View

How to evaluate or check the animation outputs (Motion) of Frequency domain response in LS-Dyna?

Dear All, I am performing the frequency response analysis of the human body FE model using steady-state dynamics. I have given base acceleration in terms of amplitude and phase angle. The output...

08 October 2023 9,735 0 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

The Bigger You Are, the Harder You Fall (some lessons from Dinosaurs)?

Evolutionary fitness is based on an organism’s ability to adapt rapidly to changing environmental circumstances. Large-bodied mammals have been equipped with large brains (and hence a high...

06 August 2024 4,849 2 View

Are air moisture harvesting technologies effective in combating desertification?

Air moisture harvesting Air water collection devices

06 August 2024 5,473 2 View

I am considering injecting cisplatin directly into an equine sarcoid tumour that is chronically refractive?

My case has been under treatment now for 3 years with little response. Now considering euthanasia. Last ditch is Cisplatin for dilution in iv infusion (100ml/ml in saline and mannitol). What can I...

05 August 2024 1,835 0 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

The question is how to use Wavenet transform?

HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?

03 August 2024 7,829 0 View

State of art in natural disasters?

Are increasing the costs of disasters in the affected countries.

01 August 2024 1,794 2 View

How can I get my Granzyme B flow cytometry stain to be consistent?

I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...

01 August 2024 7,677 2 View

Mushtaq Ahmad

may be useful for some extent

http://link.springer.com/article/10.1007%2FBF02860648#page-1

https://www.researchgate.net/publication/262604165_Process_for_mannitol_extraction_from_seaweed

may be useful

Patent Process for mannitol extraction from seaweed

Jackson Achankunju

Dear Dr.Vaibhav,

Hope the attached paper is helpful.

JABBAR MIAN AND ELIZABETHPERCIVAL, CARBOHYDRATES OF THE BROWN SEAWEEDS Himanthalia lorea,Bifurcaria bifurcate & Padina pavonia PART IL. EXTRACTION AND FRACTIONATION