Is that the same procedure(ag particle extraction) for Fe and Zn particles extraction of leaves

Send detailed procedure

Dear Ramya Krishnan

Hi, extraction nanoparticles (e.s IronOxide particles) from plants are should be interesting filed. i was interested it. please send me your procedure extraction or other procedures if its possible for you.

Thanks a lot

Nejadmoghaddam, Iran

I want detailed procedure for extraction and identification of nanopartcles from microbes

Which microorganism do you want to extract from?

Actually am to new to nanotech... I ll post the procedure which i got.. Recommend me if u know any other procedure

Synthesis of Gold Nanoparticles:

1. Add 20 mL of 1.0 mM HAuCl4 to a 50 mL beaker or Erlenmeyer flask on a stirring hot plate. Add a magnetic stir bar and bring the solution to a rolling boil.

2. To the rapidly-stirred boiling solution, quickly add 2 mL of a 1% solution of trisodium citrate dihydrate, The gold sol gradually forms as the citrate reduces the gold(III). Remove from heat when the solution has turned deep red or 10 minutes has elapsed.

3.The presence of a colloidal suspension can be detected by the reflection of a laser beam from the particles.

4. Because a laser pointer emits polarized light, the pointer can be oriented such that the beam appears to disappear. 5.When the beam from the laser is visible in one view, it is invisible in the view perpendicular to the first.

Put a small amount of the gold nanoparticle solution in two test tubes. Use one tube as a color reference and add 5-10 drops of NaCl solution to the other tube. Does the color of the solution change as the addition of chloride makes the nanoparticles closer together?

lllry procedure

1.Appropriate amounts of chloroauric acid and TX-100solutions were taken in 4 × 1 cm stoppered quartzcuvettes for photo-irradiation.

2.The cuvettes were placed at a distance of 3 cm from the light source and

were irradiated for 25 min.

3.The photoactivation produced pink colored Au(0) particles. These particles

were used as ‘seeds’ for further growth. Then various amounts of ‘seed’ particles and the Au(III) ions

were taken in such a way that the total amount of gold remains constant whereas gradually [Au(III)]/[seed]

ratio increases. The ratio was varied from 2 to 100.

4.In the next step, ascorbic acid was added slowly to the unstirred mixture of ‘seed’ and Au(III) ions. The particles develop within a few minutes.

The kinetics of particle development was followed at λ = 532 nm at 28 ± 1◦C. UV–visible spectroscopy using 1 cm quartz cuvette with UVspectrophotometer. Transmission electron microscopy (TEM) were used to characterize the particles. Sample was prepared by placing a drop of solution containing nanoparticles on a carbon-coated Cu grid. TEM measurement was carried at 400 kV .