I am wondering if anyone is aware of protocols for extracting DNA (Mitochondrial and nuclear) from formalin fixed invertebrate tissue? Has any of these methods worked repeatedly?
Hi,
The success of DNA extraction (and subsequent PCRs etc) from formalin fixed samples depends very much on if they were fixed in buffered neutral formalin or just in x% formalin. If your samples were not in buffered formalin then you are much less likely to get decent lengths of intact DNA out of them.
If in 10% NBF then the formalin pre-treatment step in a kit like Qiagen's DNeasy range may work (even if you don't use their kit you can try the pretreatment step from the kit manual).
Good luck.
Hi, this publication may help you: http://www.ncbi.nlm.nih.gov/pubmed/22237525
And: https://www.researchgate.net/post/Can_anyone_suggest_a_protocol_for_extracting_DNA_from_formalin_fixed_wax_embedded_tissue1
Cheers, Nadine
Hi,
The success of DNA extraction (and subsequent PCRs etc) from formalin fixed samples depends very much on if they were fixed in buffered neutral formalin or just in x% formalin. If your samples were not in buffered formalin then you are much less likely to get decent lengths of intact DNA out of them.
If in 10% NBF then the formalin pre-treatment step in a kit like Qiagen's DNeasy range may work (even if you don't use their kit you can try the pretreatment step from the kit manual).
Good luck.
we have extracted dna (for COI) from 100 y.o. formalin and alchogol samples. fragments were rather short (250 bp), so we had to elaborate additional primers.
Article Fixing Formalin: A Method to Recover Genomic-Scale DNA Seque...
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