please recommended me How much seeds are required for each replications? and which is suitable methods in which condition seeds can be easily investigate such as pot , hydroponic and field conditions?
parameters , seed rate, seed density etc let me know the detailed and suitable methods
thanks in advance
Drought resistance in maize can be evaluated through water stress imposition, measuring physiological traits, root phenotyping, and molecular markers associated with drought resistance. A combination of these approaches can help identify maize varieties that are more tolerant to water stress.
Root phenotyping, measuring the difference in root FW, shoot FW, leaf water potential etc... between well-watered amd water-deprived maize plants can help
Big difference implies drought sensitivity and small difference may imply drought tolerance
You require at least 3 pots per replication to obtain variance. A replicate is not a pot, it is a group of pots, each separate from each other to avoid confounding and bias. You require at least 30 seeds at each stage to fulfil the CLT :)
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