No. But you will need ethical approval from your institution

No Strictly No. Need ethical approvals if it really a absolute nuisance

Sounds horrible. I hope you are not doing it.

So there is no acceptable method using alcohol (isopropyl, ethanol etc) for euthanizing a small reptile? Including ICo administration? How about the MS-222 is it still acceptable?

And sorry for the bad english in the question, i mean "i planned to use"

From the AVMA Guidelines for Euthanasia - 2013

S7.3 CAPTIVE AMPHIBIANS AND REPTILES

S7.3.1 Anatomy and Physiology

Amphibians and reptiles include caecilians (order

Gymnophiona), frogs (order Anura), salamanders

(order Caudata), snakes (suborder Serpentes), lizards

(suborder Lacertilia), crocodilians (order Crocodilia),

and turtles and tortoises (superorder Chelonia). Once

again, these taxonomic groups differ substantially anatomically

and physiologically from each other, as well

as from mammals. Of particular concern for amphibians

and reptiles are differences in metabolism and high

tolerances to hypoxia, as compared with mammals, that

limit the effectiveness of methods based on anoxia. In

addition, consistent access to the vasculature can be

challenging and, therefore, many conventional methods

of euthanasia are less effi cacious for these species.

Because it is often diffi cult to confi rm that an amphibian

or reptile is dead, the application of two or more euthanasia

procedures is usually recommended.552,589–591

Our understanding of amphibians’ and reptiles’

nociception and responses to stimuli is incomplete;

therefore, many recommendations for minimizing pain

and distress are extrapolated from information available

about mammals. Where uncertainty exists, erring

to proactively alleviate potential pain and suffering is

recommended as an appropriate approach to euthanizing

amphibians and reptiles. Consulting multiple

references on amphibian and reptile euthanasia is advised

as a means of identifying methods that are most

appropriate for a given species and set of circumstances.

166,312,401,552,553,589–591

S7.3.2 Restraint

Physical restraint—Manual restraint is possible for

many species. Equipment may be required for restraint

of some species in some situations (eg, venomous species).

Multiple people may be required for larger species,

and at least one additional person should be available

for emergencies. Large animals may represent a

proportionately greater risk for personnel.

Chemical restraint—Chemical restraint may be

useful in some situations, particularly for venomous or

large animals where human safety would be compromised

by manual restraint. Chemical restraint at high

doses may serve as a fi rst or preparatory step of euthanasia

in some situations.

S7.3.3 Verifi cation of Death

Methods used to verify death in mammalian species,

such as auscultation, ECG, Doppler ultrasound,

or pulse oximetry, can be used for amphibians and reptiles,

but it is important to remember that amphibian

and reptilian hearts can beat even after brain death.

Death should always be confi rmed by physical intervention.

S7.3.4 Acceptable Methods

S7.3.4.1 Noninhaled Agents

Injectable agents—Venous access for administraAVMA

Guidelines for the Euthanasia of Animals: 2013 Edition 77

tion of euthanasia agents can be challenging for some

species. Intracoelomic, subcutaneous lymph spaces,

and lymph sacs are acceptable routes of administration.

Direct injection into the brain through the parietal eye,

while under anesthesia, has been described for some

lizard species.592

Sodium pentobarbital (60 to 100 mg/kg of body

weight) can be administered IV, intracoelomically, in

the subcutaneous lymph spaces, or in the lymph sacs,

although doses vary by species.593 Doses as high as

1,100 mg/kg (500 mg/lb) of sodium pentobarbital with

sodium phenytoin administered intracoelomically may

be required for euthanasia of some species such as X

laevis.312 Time to effect may vary, with death occurring

instantaneously or up to 30 minutes later.77,552,589–591,594

Barbiturates are best administered intravascularly to

minimize the discomfort upon injection.595 However,

where intravascular administration is not possible or

its benefi ts are outweighed by distress imposed by additional

restraint, pain from alternate methods, risk to

personnel, or other similar reasons, intracoelomic administration

is an acceptable route for administration

of barbiturates.

Dissociative agents such as ketamine hydrochloride

or combinations such as tiletamine and zolazepam;

inhaled agents; and IV administered anesthetics, such

as propofol, or other ultra–short-acting barbiturates,

may be used for poikilotherms to induce rapid general

anesthesia and subsequent euthanasia, although application

of an adjunctive method to ensure death is recommended.

External or topical agents—Buffered tricaine methanesulfonate

(MS 222) may be administered via water

baths (amphibians), or injected directly into the lymph

sacs (amphibians) or the coelomic cavity (amphibians

and reptiles).596–599 Prolonged immersion (as long as 1

hour) may be required for 5 to 10 g/L water baths.312,593

Tricaine methanosulfonate does not create histopathologic

artifacts.596 See the Noninhaled Agents section of

the Guidelines for additional information.

Benzocaine hydrochloride, a compound similar to

MS 222, may be used as a bath or in a recirculation

system at concentrations  250 mg/L or applied topically

to the ventrum as a 7.5% or 20% gel for euthanasia

of amphibians.600 A dose of 182 mg/kg of benzocaine

gel (20% concentration, 2.0-cm X 1.0-mm application)

has been reported as effective for euthanasia of adult

X laevis.312 Pure benzocaine is not water soluble and

should be avoided for anesthesia or euthanasia because

it requires the use of acetone or ethanol solvents, which

may be irritating to tissues.310

In general, these noninhaled agents are highly effective,

their onset of action is rapid, and they are applicable

across a range of species and sizes of animals.

However, general anesthesia may be required prior to

administration, some require IV administration for vessels

that may be diffi cult to access, they may produce

undesirable tissue artifacts, a controlled substance license

is required for barbiturates and some other products,

and there may be environmental pollution and

toxicity concerns depending on method of disposal of

the remains.

S7.3.5 Acceptable With Conditions Methods

S7.3.5.1 Inhaled Agents

Inhaled anesthetics—Inhaled anesthestics are acceptable

with conditions when they are more practical

than the previously mentioned acceptable methods,

and where the limitations of this method are understood

and addressed. Many reptiles and amphibians are

capable of breath holding and shunting of their blood,

which permits conversion to anaerobic metabolism for

survival during prolonged periods of anoxia (up to 27

hours for some species).601–606 Because of this, induction

of anesthesia and time to loss of consciousness may be

greatly prolonged when inhaled agents are used. Death

may not occur even with prolonged exposure.552,589–591

Lizards and most snakes do not hold their breath to the

same extent as some of the chelonians, and are therefore

more likely to have a clinical response to inhaled

agents. Regardless of the species or taxonomic group,

death must be verifi ed prior to terminating the use of

the inhaled agent, or a second, guaranteed lethal procedure

(eg, decapitation) should be performed to ensure

death.

Inhaled anesthetics are effective, have a moderately

rapid onset, appear to induce a painless death, can maximize

use of the euthanized animal for analytic studies,

and can minimize the need for animal handling. Caveats

include that inhaled anesthetics are most suitable

for smaller species, animals may experience an excitation

phase prior to becoming anesthetized, they present

environmental pollution and occupational hazard concerns,

some are irritants or are perceived as noxious,

and amphibians and reptiles may be resistant to their

action because of breath holding.

Carbon dioxide—Carbon dioxide may be considered

for euthanasia of amphibians and reptiles if alternate

methods are not practical and where the limitations of

this method are understood and addressed.401,552,553,589–591

Due to the potential lack of response to this method

by many species and the requirement for a prolonged

exposure time, other methods are preferable. Death by

CO2 must be verifi ed, and preferably, assured by application

of a secondary lethal procedure.

S7.3.5.2 Physical Methods

Penetrating captive bolt or fi rearm—Crocodilians

and other large reptiles can be euthanized by a penetrating

captive bolt or gunshot (free bullet) delivered to

the brain.166 Line drawings of the head of various amphibians

and reptiles, with recommended locations for

captive bolt or fi rearm projectile penetration, are available.

401 Refer to ballistics details in the Physical Methods

section and experts for more information on selection

and use of fi rearms.

These methods are moderately rapid (allowing for

restraint), are applicable across a wide range of species

and sizes, and leave no environmental residues other

than lead (in the case of free bullet), which can be sequestered.

However, size-appropriate equipment and appropriately

trained personnel are required, violent muscle

contractions can occur following their application,

78 AVMA Guidelines for the Euthanasia of Animals: 2013 Edition

and they may be aesthetically unpleasant for onlookers.

Manually applied blunt force trauma to the head—

This method is acceptable with conditions, when other

options are unavailable, as long as it is performed by

well-trained and skilled personnel and if an adjunctive

method, such as decapitation or pithing, is promptly

applied to ensure death.52,132,589,591 Further research is

needed to clarify methods, taxa, and size ranges where

this method is effective and humane.

Rapid freezing—Reptiles and amphibians can be

euthanized by rapid freezing when it results in immediate

death. Based on rodent models, it is likely that this

can be achieved by placing animals < 4 g (0.1 oz) in

liquid N2.52 The technique should not be used for species

that have adapted freeze tolerance strategies, as this

method may not result in instant death.607 Placement of

animals  4 g in liquid N2 or other uses of hypothermia

are not acceptable.

S7.3.6 Adjunctive Methods

Decapitation—After animals have been anesthetized,

decapitation using heavy shears or a guillotine

is effective for some species. It has been assumed that

stopping blood supply to the brain by decapitation

causes rapid loss of consciousness. However, because

the CNS of reptiles and amphibians is tolerant to hypoxic

and hypotensive conditions,401 decapitation must

be followed by pithing or another method of destroying

brain tissue.589,591,595 Decapitation should only be performed

as part of a 3-step euthanasia protocol (injectable

anesthetic, decapitation, pithing).

Pithing—Pithing can be used as a second-step euthanasia

method in unconscious animals when performed

by properly trained individuals.589,591 The pithing

site in frogs is the foramen magnum, and it is identifi ed

by a slight midline skin depression posterior to the skull,

midline between the eyes, with the neck fl exed.552,590

S7.3.7 Unacceptable Methods

Hypothermia—Hypothermia is an inappropriate

method of restraint or euthanasia for amphibians and

reptiles unless animals are suffi ciently small (< 4 g)52

to permit immediate and irreversible death if placed

in liquid N2 (rapid freezing).589,591,595 Hypothermia reduces

amphibians’ tolerance for noxious stimuli608,609

and there is no evidence that it is clinically effi cacious

for euthanasia.610 In addition, it is believed that freezing

can result in the formation of ice crystals in tissues that

may cause pain.52,401 Consequently, because amphibians

and reptiles lack behavioral or physiologic means of

demonstrating pain or distress while hypothermic, generalized

prohibitions on hypothermia for restraint or

euthanasia are appropriate. Localized cooling in frogs

may reduce nociception, but this localized effect is not

appropriately applied to the whole body as a part of euthanasia

procedures.611 Freezing of deeply anesthetized

animals may be justifi ed under circumstances where

human safety could be compromised.612

S7.3.8 Special Cases and Exceptions

Intracardiac administration of euthanasia agents is

acceptable for captive amphibians and reptiles that are

unresponsive to stimuli because of disease or the application

of other euthanasia methods, or in cases where

other routes are not possible.

Neuromuscular blocking agents may be used for

routine anesthetic procedures of crocodilians and some

other taxa and are, therefore, considered acceptable

with conditions for restraint of reptiles if given immediately

prior to administration of a lethal agent. These

agents are not acceptable as a sole means of euthanasia.

Injectable agents such as lidocaine hydrochloride,

potassium salts, or magnesium salts may be useful as an

adjunctive method to prevent recovery.591

Perfusion with fi xative of a deeply anesthetized animal

can be used to euthanize amphibians and reptiles

when scientifi cally justifi ed.