Hi
So I'm trying to grow various microbes into liquid broth with the presence of bio-charcoal... I'm hoping that since charcoal has a relatively large compartments that microbes can enter within it so that I'm able to observe them under SEM.
I want to induce the biofilm formation of these microbes within the charcoal so that they adhere to the charcoal walls using it.... any ideas on how to better enhance the biofilm formation ability?
Some of the bacteria that I'll be using:
Bacillus, E.coli, P. aeroginosa and S. aureus
Cheers
yes, you can do so. May be you put your culture on shakers for 2-3 days, that will give good growth.
Application of Biofilm-Covered Activated Carbon Particles as a Microbial Inoculum Delivery System for Enhanced Bioaugmentation of PCBs in Contaminated Sediment
http://www.serdp.org/Program-Areas/Environmental-Restoration/Contaminated-Sediments/ER-2135/ER-2135
You can alter the composition of your liquid broth. For example, P. aeruginosa will form more robust biofilms under magnesium limitation or after the addition of eDNA (PLoS One. 2011;6(8):e23307. If you are using a general purpose broth, like tryptic soy broth (TSB), you can simply dilute it 1:20.
Article Magnesium Limitation Is an Environmental Trigger of the Pseu...
I agree with Amber J Park. Dilution of broth, or any other stress treatment (high temperature for 2-4 hrs, nutrient dilution, stationary phase, cation deficiency etc) will induce biofilm formation. eDNA addition will enhance the integrity of matrix. sugar will enhance EPS
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