Hello Dhaval, usually the easiest method is to use a cytospot, which is basically a centrifuge in which you can fit special chambers hosting a glass slide and your cell suspension solution separated by a membrane. The cells will pass the pore in the membrane and be shot on the glass slide, sticking to it. You can run the H&E staining or other antibody stainings directly after centrifugation.

The technique for mono-layer preparation in cytocentrifuge might be useful. Then you fix the mono-layered glass slide or cover-slip and follow H&E protocol.

Thanks.. actually it worked, when tried PMA  the cells were dying.

Refer this article. A Simple and Efficient Method for Preparing Cell Slides and Staining without Using Cytocentrifuge and Cytoclips for suspension cells

https://www.hindawi.com/journals/ijcb/2015/813216/