Take TLC for each plant separately do know the number of components and their Rf.

Then take TLC for mix. three plants together

mix the mix. with honey and boil.

Finally take TLC for last mix. and compare the Rf , if you get exactly the Rf it mean no chaining but if some disappeared and appear new spots it means some reaction or coordination took place between the components.

thank you very much dear brother Faiq H S Hussain ..

But does the GC-mass test work?

It is possible to use GC-MS before and after mixing and compare the results

Dear Faiq H S

Thank you very much my wonderful friend .. My appreciation and respect to you.

Dear all, can you provide monographs of Hygrophilla auriculata, Chenopodium album/opulifolium, Ttopaeoluma majus, senna septentrionalis, Celosia trigyna, Ocimum gratissimum, Cymbopogon cittratus, Hypoestes triflora/Dicliptera colorata. An suitable solvent for crude extracts. Thank you.

TLC, HPLC, GC-MS