What are you trying to do with the extract in mice? not clear. Inject?

Seems to me the EtOH is quite nonpolar, so water did not work to re-dissolve.

I worked on a project once where sesame oil was used as vehicle for injection of crude drug substance into mice.

Matthew Bernart Thank you for your answer. I'm trying to take the extract for mice by PO (oral). But The final extract is solid form. I want to change liquid form by solvent which mice can take by PO. With condition, solvent is not toxic for mice

sesame oil would not be toxic per se, but there would be a limit beyond which digestive difficulties would be encountered which would threaten the reproducibility of your experiment.Perhaps if you did not take the extract to complete dryness on the rotavap, you could more easily mix it into a vehicle that the mice could tolerate- When you find a suitable vehicle, try the blank vehicle on the mice and record observations. Good luck!