I try to dissolved three kind of plant extracts in 100% DMSO before I test them with the cell lines. The methyl extract is easily dissolved, but the hexane and ethyl acetate extracts are poor. I know that hexane extract mostly contain non-polar compounds, so I think I will use other non-polar solvents to dissolve it, like toluene or hexane but I don't have any idea for ethyl acetate extract. How can I solve this problem?
Thanks in advance.
compounds extracted in Ethyl Acetate should be soluble in DMSO.
Do you have completely dried the extracts?
DMSO will be the solvent of choice. As suggested by Vijay it should be dissolving in DMSO. Kindly make ur extract completely dry.
In our lab, we usually evaporate all the solvent first then we dissolve the residue in DMSO. Sometimes it takes some time to dissolve or may require using the vortex for a longer time. If your extract didn't dissolve completely, I suggest you check the literature to get an idea about what to expect in your extract.
I already had to dissolve extracts of plants carried out with the Ethyl acetate in DMSO and solubility was good.¶What I recommend to you, perform again your extraction andyou ensuring that you well had filtered and dried completely the extract with evaporator
The extract dissolves better in DMSO if it had been filtered. You could also apply the tube containing the DMSO mixture to a warm water bath.
To perform a good solubilization the extracts must be completly dry.
I had the same problem with an AcoEt extract so the solubilization in water (1% w/v) is correct using a warm waterbath (35-40°C) with an ultrasonic bath. It takes a long time (15 minutes). The solution is stable when it comes back to ambiant temperature. You can observe a precipitate after two or three days.
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