I have isolated few bacteria in MRS agar (pH 6.2), Starch Casein Agar (pH 7.2) and LB agar media (pH 7.2) but I am confused how to differentiate each other. Kindly give some suggestion.
Lactic acid bacteria group of microorganisms characterised by the production of lactic acid as the main metabolic end product from glucose and reducing pH. First check strains they should be gram-positive and catalase negative than after inoculation in MSR broth, you should see significant decrease in pH after 48 hour, which you can measure by pH meter. Further biochemical identification and Carbohydrate fermentation you can perform by Api test.
I think it will be better to use molecular tools, that are very fast and give you an exact results.
The molecular techniques will solve this problem. API may add some information
Dear Ajitti,
As per Dr. Moustafa Y. El-Naggar Suggestion , you can use API 50 CHL Medium in the Differentiation of Lactic Acid bacteria .
API 50 CHL Medium, intended for the identification of the genus Lactobacillus and related genera, is a ready-to-use medium which allows the fermentation of the 49 carbohydrates on the API 50 CH strip to be studied.
Hello, APi test is better to use after fulfilment culture belongs to LAB or not. As I see the question was differentiation of LAB from other Bacilli. API test is used for identification of Lactobacilli in subspecies level. The use of API test just for differentiation LAB from other bacilli is very expensive and time wasting way.
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