I want to know the toughness of porous scaffold. What means can it work for this?
I plan to do transposon mutagenesis in an anaerobic Gram positive bacteria. The easiest way for transposon mutagenesis is the commercial Tn5 kit. I used that for E. coli before. But I didn't see...
11 September 2023 8,530 2 View
I am trying to use double selection marker, G418/kanR and BleoR in pPICZalpha plasmid. I am constructing the plasmid with both antibiotic resistance gene and clone it into E. coli Top10. However,...
04 July 2023 288 5 View
I am adding two genes into the same plasmid. I plan to make it as promoter1-gene1-terminator1--promoter2-gene2-terminator2. Does anyone know how many bp interspace should I leave between...
15 May 2023 2,126 3 View
Hello! I am using T7-mcherry plasmid in E. coli BL21(DE3). When I streak it on LBAmp plate, the colonies show beautiful purple-red color. When I streak it on LBAmp+0.1mM IPTG plate, the colonies...
26 April 2023 6,918 2 View
I recently ran into difficulties. After feeding FITC-labeled drugs to the insects, the insects' intestinal tissues were sectioned and then observed using fluorescence microscopy. However, we found...
29 June 2022 5,955 0 View
May I know why commercial sintering die attach paste is always based on silver? Why not other metals, such as copper and aluminum? Thanks.
18 March 2022 7,452 4 View
Hi, May I know what is the industrial process to coat copper wire with palladium in the production of PdCu wire? What is the process and what instruments are used? Please kindly advise. Thanks.
18 February 2022 807 5 View
How to measure moisture diffusion coefficient for plastic materials?
19 December 2021 5,892 3 View
Does light have a possible shortest limit of wavelength and a possible longest limit of wavelength? When the wavelength of light becomes very very short, i.e., infinitely short, or infinitely...
28 June 2021 9,321 5 View
Zero Check?
26 June 2021 3,017 2 View
Hello everyone, I recently submitted an article to Geotechnical Engineering (Proceedings of the ICE), and the current status has been listed as "EiC Pre-assessment: Ready" for the past 20 days. I...
10 August 2024 6,493 1 View
I need to model an anisotropic material in which the Poisson's ratio ν_12 ≠ ν_21 and so on. Therefore, the elastic compliance matrix wouldn't be a symmetric one. In ANSYS APDL, for TB,ANEL...
09 August 2024 5,048 2 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...
06 August 2024 726 3 View
I am working on Abaqus/Explicit(Quasistatic ) for the deformation of the auxetic structure model. Please explain how the plastic input value should be considered from the true stress-strain curve...
05 August 2024 454 3 View
What information we can get from PXRD analysis other than from SCXRD analysis of a crystal ?
30 July 2024 6,261 4 View
What should a Mechanical Engineering PhD scholar focus on during their PhD to enhance their chances of securing a postdoctoral position?
29 July 2024 7,714 4 View
In some data sources it has been grouped in Q1 and some shows it is Q2.
29 July 2024 4,227 2 View
How to apply magnetic field parallel to b axis of any crystal
29 July 2024 4,083 2 View
Dear Research Community I would like to invite Elctrical Engineering specialists to solve problems related to Ternary Algebra or tripple sets I have an algorithm that places in relation three...
28 July 2024 5,771 2 View