I would like to determine the proportion of every constituent of my essential oil.
I made GC MS and the the qualitative analysis by recognising the different constituents presents using n-alkanes series. And now I'm looking for a way to determine the proportion of my constituents.
Any help in this regard will be appreciated.
Hi Alexander,
you can determine it using the peak areas of the costituents, calculating for every compound the relative peak area. It would be better to use peaks of GC-FID for relative "quantification". However, in literature, it is quite common to use also the peaks areas from GC-MS (which generally just slightly differ from FID detector for this kind of extracts).
If you know exactly the substances that make up your oil! You can use them as a reference and deduce the % of the constituents of your oil by making the ratio between the area of the peaks
It is usually, the area below of the absorption peak is calculated based on the mathematical relations and the programming algorithm of the device software and the relevant value is reported based on percent, too. In order to find the accurate value of each compound/analyt should be used from the calibration curve coupled with determining the retention index of each unknown compound (the isometrical analysis).
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