I do not know if i could use this formula:
number of point per peak= peak width of 10 seconds/(dwell time (s) X number of MRM events)
(MRM-Multiple Reaction Monitoring).
where i can found a document with the discription of the calculation?
If your peak is 10 seconds wide then the formula is basically correct. If you are using a settling or pause time between the MRM transitions then you need to add this time to the dwell time, visit: http://www3.appliedbiosystems.com/cms/groups/psm_marketing/documents/generaldocuments/cms_050558.pdf
http://www3.appliedbiosystems.com/cms/groups/psm_marketing/documents/generaldocuments/cms_050558.pdf
While your formula is correct, you are doing it backwards. The point of this calculation is not to calculate how many datapoints per peak you end up with, but rather how long your dwell time should be to obtain a set number of points per peak.
This is the formula you should be using:
Dwell = pw / (n * mpx) - pt
With Dwell = dwell time in seconds, pw = peak width in seconds, n = datapoints per peak, mpx = amount of simultaneous targets (multiplexing), pt = pause time in seconds.
I would advise you to take about 10 datapoints per peak.
So, with a peak width of 10 seconds, 10 datapoints per peak, 5 simultaneous targets, and a pause time of 50 ms, your maximum dwell time becomes:
Dwell = 10 / (10*5) - 0.05 = 0.15 s ( =150 ms)
Note that all mass spectrometers need a little bit of time to switch between transients. You need to check your instrument manual for this so called "pause time" as it can differ between instruments and settings.
Good luck!
Good afternoon Doctor Bruce
Thank you for your answer and for help me in ths question.
I will study the document that you send it
Best regard
Joaquim
Good afternoon Alexander Streng
Thank you for your answer and for help me in ths question.
i will check the equipment and look in the manual
Best regard
Joaquim
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