I need to determine the content of pederin in the brazilian species of Paederus rove beetles (Coleoptera, Staphylinidae). What the best method? Can I estimate the content analysis in lesioned experimental animals or should I use a high performance thin layer chromatography (HPTLC) as other researchers do? The first method is more feasible for me. I can estimate the content of pederin for each species based on the characteristics of the lesions. Is this okay?

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