urfactant for the preparation of NLCs was selected based on its capacity to emulsify solid–liquid binary lipid (SLB). 100 mg of SLB
was dissolved in 3 mL of methylene chloride and added to 10 mL of 5% surfactant solutions under magnetic stirring. Organic phase was removed at 40 ◦ C and the resultant suspensions were diluted with 10-fold Milli-Q water. Percentage transmittance of the resultant samples was observed using UV spectrophotometer at 510 nm.
On what basis 510 nm was chosen?