hai, i'm new in fisheries field. can someone share with me the protocol of determining ageing and migration of fish using otolith structure. Thank you in advance
I will be using Lipofectamine 3000 for plasmid transfection in A549 cells. I plan to test different concentration of plasmid that can give best result. so i decided to test 1ug, 2.5ug and 5ug of...
02 March 2017 2,141 1 View
hi i'm doing lentiviral transduction A549 cell line. during the transduction, i removed the medium containing serum and replace it with serum-free medium and added appropriate amount of virus....
01 February 2017 5,160 14 View
I've done several techniques to transfect shRNA-containing plasmid into A549. The techniques that i've done including calcium phosphate, lipo 3000, neon transfection. Now i planned to try...
08 September 2016 2,510 5 View
"I have treated adult zebrafish with 8-micron polystyrene microplastic and want to study the bioaccumulation in different organs. Can this be done using hydrogen peroxide digestion followed by...
05 August 2024 853 3 View
In the aquaculture and fish nutrition research field, a number of growth and somatic indexes are often used, including specific growth rate (SGR), feed conversion rate (FCR), protein efficiency...
22 July 2024 2,480 4 View
Chemical Engineering /Food Engineering
20 July 2024 4,152 4 View
Dear colleagues, We are trying to do FISH on mice PDEC metaphase spreads. We use homemade biotin labelled probes, synthetized using a nick translation kit. For hybridization, initially we were...
08 July 2024 8,687 3 View
I measured the fatty acids in the fish, using the internal standard method in the national standard method, I first extracted the fish oil with cable extraction, please ask me how many grams of...
02 July 2024 900 1 View
It's for my thesis on Quaternary costal sediments from Western Greece.
01 July 2024 4,854 0 View
Other than Sparids
27 June 2024 1,809 0 View
No response from fish physiology and biochemistry journal after a year of peer review. Journal editorial team is not responding even for Request for withdrawal of our manuscript. We are struck...
25 June 2024 8,918 0 View
Hi, I´d like to trace the C and N flow in a recirculating aquaculture system by labelling the fish feed with concentrated stable isotopes. From a supplier, I found a product ouf of algae lysate...
19 June 2024 746 0 View
Could someone please confirm if those vacuolated structures are goblet cells? And whether the sections exhibit signs of inflammation. Context: I am conducting histological analysis of the...
15 June 2024 3,208 4 View