Noor hanis abu halim

4 Questions 8 Answers 0 Followers

Questions related from Noor hanis abu halim

How to determine ageing and migration of hilsa using otolith structure?

hai, i'm new in fisheries field. can someone share with me the protocol of determining ageing and migration of fish using otolith structure. Thank you in advance

04 April 2018 9,147 0 View

Has anyone used Lipofectamine 3000 for plasmid transfection in A549 cells?

I will be using Lipofectamine 3000 for plasmid transfection in A549 cells. I plan to test different concentration of plasmid that can give best result. so i decided to test 1ug, 2.5ug and 5ug of...

03 March 2017 2,118 1 View

Does anyone know why cells morphology change (cells look like dying) when i starved cells without serum during transduction?

hi i'm doing lentiviral transduction A549 cell line. during the transduction, i removed the medium containing serum and replace it with serum-free medium and added appropriate amount of virus....

02 February 2017 5,140 14 View

What are the best parameters for electroporation of A549?

I've done several techniques to transfect shRNA-containing plasmid into A549. The techniques that i've done including calcium phosphate, lipo 3000, neon transfection. Now i planned to try...

09 September 2016 2,498 5 View