In my lab we use every 4th section of the mouse striatum to count GFP-TH+ neurons. The distribution of these neurons is linear in the rostrocaudal direction.
I found the Koenigsmark formula (1970), which allows you to determine the total number of neurons in the entire striatum using non-serial sections:
Nt = Ns* St / Ss, where:
Nt - number of all (total) neurons:
Ns - number of all neurons at the 4th sections;
St - total number of slices of the striatum;
Ss - number of slices used.
1. Do I need to use an adjustment to eliminate possible double counting of neurons when using the Koenigsmark formula?
2. Maybe someone knows other methods of approximation that would work better, more precisely?
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