Is there any miRNA fluorescent labeling kit (or methods) that does not affect (add) the length of the miRNA?

I want to label my target miRNA and see the miRNA trafficking. But I don't want to use any plasmid or radioactive label. I need the fluorescent label that will not affect the length of my...

06 December 2020 8,471 3 View

Can too much of Tris-Cl buffer inhibit PCR?

I accidentally resuspended my stock primers (100 uM) in tris-Cl (pH 8.0) 1 M instead of 10 mM. I do PCR with 20 uM dilution from the stock. Will the high concentration of Tris-Cl inhibit PCR? I...

12 July 2020 2,971 10 View

Does hot KOH react with zirconia?

Hot KOH destroy many materials like alumina or glass.. So, can I use zirconia(ZrO2) boat for KOH activation of carbon nanoparticle experiments?

09 April 2020 800 1 View

Paired t-test or Independent samples t-test?

Hi, I am working on the home advantage. And examining the difference of a player's performance between playing at home and away. However, the sample size is different because the player played...

24 February 2020 7,053 3 View

Is it possible to wash PPFE slides that have been put through fluorescent reaction and stain them again?

I have some 3 year old PPFE slides of human brain that have been reacted with primary antibodies then reacted with Alexa Fluor secondary antibodies. The DAPI on the slides is still visible but the...

02 May 2019 7,390 2 View

What type of statistical tests on SPSS should I use for my study?

Hi I am carrying out a study investigating the association between age and password strategies among online users. I have collected my data, and inputted it on SPSS, however, I am not sure which...

10 December 2018 1,002 7 View

Can docking be used to predict a peptide's target receptor?

Let's say I have an array of receptors. Given a peptide, can protein-peptide docking be used in predicting the peptide's target among the receptors? If so, how? If not, why? Can you recommend me...

24 July 2018 3,569 4 View

Fc block not working for Flow Cytometry?

Good day all! I would greatly appreciate help with the following issue: Our lab is trying to characterize mouse lymphocytes for Tbet, EOMES, and PD-1. When I ran the samples the first time, the...

28 March 2018 1,225 3 View

In general speaking, how long can I keep antibody-labeled sections in wash buffer after the staining of secondary fluorescent antibody?

If the mounting is delayed for a day or two, would the specific binding being greatly reduced?

05 December 2017 4,103 3 View

Call for Papers: Australian Gender Economics Workshop (AGEW), Feb 2018, Perth Australia ?

15 October 2017 9,870 4 View

12% ledeburitic cold work tool steel, etched for revealing retained austenite, any help for clarifying?

Hello everyone. Given microstructure image belongs to a 12% ledeburitic cold work tool steel (annealed and tempered). The sample was etched with picral (100 mL ethanol and 4 g picric acid). I...

02 March 2021 4,119 3 View

Physical-chemical formation of stalactites/stalagmites. what promotes the formation/growth of these structures?

I know, or think I do, the basic idea behind these natural structures, as from what I know they form due to water dripping from the top leaving a salt deposit behind. But started to wonder about...

22 February 2021 1,400 4 View

Is there an ideal incubation time of RGO-modified SPCE before subsequent use, i.e., electrochemical characterization or further modification?

I notice that upon washing and air-drying of my RGO/SPCE surface and characterizing again via DPV, the current response towards ferricyanide probe significantly decreases. My worry is that the...

22 February 2021 5,324 3 View

How can I measure the surface interaction or surface interfacial chemistry between polishing abrasives and upper surface of substrate ?

I am working on polishing abrasives. I need to do few characterizations ? which can provide the on time surface chemistry of substrate with polishing abrasive during optical polishing. I would...

18 February 2021 5,319 3 View

Despite single-cell RNA, how can I characterize a new cell subtype/phenotype?

A new cell subtype was found out only during regeneration phases after a cuprizone-induced demyelination model. How can I characterize this cell? It is important to highlight that was already...

13 February 2021 1,245 1 View

How can I spin a thick layer of organic polymer?

I need to spin a 120um thick film. My current procedure consists of spinning the polymer (about 20um), uv exposure, plasma etch, and then spinning another 20um thick layer, uv again, plasma etch...

10 February 2021 2,055 6 View

How to model in HFSS or FEKO Free Space Material Characterization?

I am having trouble setting up this problem in HFSS or FEKO. In HFSS, I've tried both FEM and FEBI-IE solvers. With FEKO, I've tried it's MoM solver and default settings. I am would like model...

09 February 2021 375 2 View

Is it possible to find out the wettability and contact angle of water on the rough or corrugated substrate?

First of all, whether it is possible to identify the presence of water and its amount on the Silicon substrate by using only FT-IR spectroscopy. Can ATR-FTIR spectroscopy find out the wettability...

03 February 2021 9,085 4 View

How do microbes tell the presence of an inorganic minaral in the soil?

Due to their ubiquitous nature, there is a tendency of the presence of microbes in a given mineral(s). For instance, if microorganisms that love the of pH less than 6 in the pH value, is isolated...

03 February 2021 4,630 10 View

In your view, which current SEM is most recommended for EBSD/TKD and EDS analysis?

I know that this is a very difficult question, as the areas of application can vary greatly. However, if you want to operate low as well as high resolution (from a few millimeters to nanometers),...

02 February 2021 4,985 4 View