How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

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03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

Logistical regression using SPSS for a dichotomous dependent variable, with an independent variable of 1, 2, 5 and 10 seconds?

I have an experiment with 28 participants The independent variable is the times 1, 2, 5, and 10 used as the cross hairs during a task These 4 times were tested using 4 different blocks, each...

03 March 2021 9,692 2 View

How was your feeling, when your first research paper was published ?

Well, writing a research manuscript is an intimidating process for many new writers and one of the stumbling blocks is the beginning of the process and creating the first draft. Please share your...

29 December 2020 5,180 15 View

What is the best way to make executable stand-alone app with simulation and visual programming interface for kids?

Everyone knows matlab simulink, modelica and so on for simulation calculations. I want to make stand-alone app for kids with visual programming interface dor simulation different process and...

02 October 2020 3,491 3 View

Hierarchical regression analysis in spss?

I run a hierarchical regression in SPSS with two IVs (2 blocks) and one DV. I first enter my first IV and to see its unique contribution to my DV. then I enter my second IV to see whether there...

14 August 2020 9,448 1 View

What is the proper way to analyze the structural response due to excitation?

Hi all! I have a complicated composite specimen vibration velocity response due to shaker excitation. The excitation signal consists of 3 predominant short impulses with small time separation...

03 August 2020 2,333 3 View

Nonparametric equivalent to ANOVA block design or two way anova?

Hello everybody. I am dealing with the analysis of an experiment where I want to compare a variable after measuring it in several cells of three plants per treatment. My hypothesis is that the...

18 July 2020 7,015 2 View

2D rotating detonation simulation - How to implement ANSYS UDF boundary condition?

Hi everyone, I am doing 2D rotating detonation engine (RDE) simulation in ANSYS Fluent. For pressure inlet boundary condition, there are three possible cases: 1. Pi > Po, where Pi is the...

25 May 2020 7,040 16 View

How to apply DWT over each non-overlapping block of grayscale image?

I want to decompose a grayscale image of 512*512 in 64 nonoverlapping blocks of size 64*64. After that, i want to apply 1 level of DWT on each of 64 blocks. Further need the LL component of each...

20 May 2020 7,163 1 View

Question for SAS with two Factor?

I have experimented with two Factor: Factor A: Two iron sources and Factor B: two seasons with three blocks. When I run the SAS, the interaction not significant, but the combination of the...

18 May 2020 2,576 6 View

Isolating Total RNA from Paraffin blocks by TRI reagent?

Dear All, I got 2 weeks old paraffin blocks of tumor masses. I want to extract total RNA from them. The available reagents in my lab are: 1) Cell dissociation buffer (GIBCO#13151-014) 2) TRI...

30 March 2020 5,067 5 View