Please explain what are the factors effect homo coupling in grignard reaction? And how to control homo coupling?
When i am trying to preparing Grignard, by R-X, in grignard dimer (R-R) formation observed in high percentage, and free Grignard R-Mg-X around 20 % observed, how to avoid the dimer formation...
11 January 2019 829 5 View
How to prepare A solvate ? what are the condition required for the Solvate formation ?is it possible to find out solvate formation nature of a compound /drug/molecule by observing its structure.
15 April 2017 5,576 1 View
how to isolate/ separate 1-(3-hydroxy-4-nitrophenyl)propan-1-one, from 1-(5-hydroxy-2-nitrophenyl)propan-1-one ? while on nitration of (3-hydroxyphenyl)propan-1-one obtaining two products those...
17 April 2016 466 2 View
how to isolate / crystallize citric acid form water ?
07 March 2016 1,459 2 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
I have used Prussian blue nanoparticles as a redox couple. The PBNPs have been made using only one salt precursor. Also, during scan rate studies, a small oxidation peak can be consistently found...
31 July 2024 9,697 0 View
Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...
31 July 2024 2,406 6 View
Can anyone with an MJ research / BioRad PCR machine from ~2010 or earlier tell me the external measurements (LxWxH) of the removable standard PCR alpha modules that can be removed from a PTC200 or...
30 July 2024 2,867 0 View
Hello all, I have been trying to follow a 2-stage PCR protocol used to amplify barcodes of a large yeast library, as per Nyugen et al. (2022) -...
30 July 2024 841 2 View
I need to install this program
25 July 2024 4,756 0 View
In general, when performing a Tafel analysis for corrosion resistance measurements, a line voltage scan is performed from say -250 to +250 mV vs. OCV at low scan speed around 0.125 mV/s. However,...
25 July 2024 7,982 3 View
I want to introduce 2 mutations using Agilent's multi-site mutagenesis kit, I designed the primers using their online tool and it gave me 2 sets for primers (1 set for each mutation) so I was...
25 July 2024 6,517 3 View