How to check normal distribution in a cell viability test?

To choose whether a parametric or a non-parametric test should be used to compare data on viability that emerge from an assay such as MTT, one should establish if the data are normally...

02 February 2021 1,733 4 View

Why I observe different growth curves in Flp-In T-REx system?

Hi, I'm currently working with the Flp-In T-REx system to get isogenic stably transfected cells expressing my GOI (gene of interest, of about 1.4 Kb). I have generated a Flp-In Cell Line and since...

17 February 2020 5,608 2 View

Why does my MS show me only base peaks?

After changing the septum of my Focus GC connected to a DSQ II MS and after a complete cleaning operation on it, my mass spectrum became completely random. It shows me only base peaks in a...

10 June 2019 481 4 View

Can anyone suggest key literature and leading institutions in the field of Energy Transition?

Dear all, My background is in Energy Economics and Political Science and I would like to improve my knowledge on the Energy Transition issues. In your opinion, what are the most relevant and...

25 October 2018 8,507 7 View

How to simulate 3D beam to 3D beam contact in ansys APDL?

Hi, I'm modeling a lattice structure and I would like to insert the contact elements between 3D beams to get the whole structure non linear deformation. Since I never used contact elements before...

22 July 2018 1,551 2 View

GNSS Carrier phase Single difference residuals?

Dear all if I have the SD carrier-phase measurements how can I compute the SD residuals? Do i need to calculate the SD residuals before fixing the integer ambiguity?

10 April 2018 7,001 2 View

Interpolation of product formation in biocatalysis using integrated Michaelis-Menten equation?

I want to interpolate the amount of product formed (as concentration or % of conversion) vs reaction time in a biocatalysis process. The fitting equation should have as (y) the amount of product...

15 January 2018 6,790 3 View

What is the main goal of modeling, and maybe of scientific knowledge tout court?

While ideally aiming at understanding an empirical phenomenon, and at the same time predicting its behaviour, scientists often focus only on one of these two aspects. Is this the dilemma of a...

13 November 2017 6,036 5 View

Are there physical applications of Banach-Tarski Paradox?

Banach-Tarski Theorem (also known as Paradox) is a mathematical statement which says that a sphere can be splitted into two (or an integer N...) spheres each equal to the original one. Do you know...

30 October 2017 9,335 2 View

What is the state of art regarding Navier-Stokes?

Which is the status of mathematical research about existence and unicity for Navier-Stokes equations (and boundary problems) for finite or infinite domains? Gianluca

17 October 2017 1,618 3 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

How to choose ANN number of hidden layers and nodes?

Hello, Could you please share any interesting research explaining how to choose the number of hidden layers and nodes per layer in case of regression problems using ANN? Thank you, any help would...

01 March 2021 6,200 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

How could I take into consideration the weights of observation in a system of seemingly unrelated regressions (SUR) in rstudio?

I build a model of 4 regression equations (probit and OLS) in R studio. I need to take the weights of observations into consideration. While it is simple to do so for every regression equation,...

27 February 2021 1,233 1 View