How to conjugate IL-6 antibody to fluorophore?

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Can someone help me with this error that I am getting while estimating a VAR model in R?

I am using annual time series data to estimate a VAR model in R. However, when I run the command to find the optimal lag length, I get the following error: Fehler in VARselect(data, lag.max = 10,...

18 February 2021 9,647 3 View

A three level hierarchical data structure in multilevel modelling, for multiple response question?

Im refering to Andy Field's example in his book Discovering statistics using SPSS, 3rd edition, page 728, where he gives an example of a data structure with level 1 variable is a memory recall...

26 December 2020 9,717 6 View

How do i handle Ti and/or Aluminium powder. Since in the instruction on the container is written that CATCHES FIRE SPONTANEOUSLY IF EXPOSED TO AIR ?

i am working on max phase preparation......i am a newly joined PhD scholar. I wanted to make MAX phase of Ti3AlC2 , in most of the literature they are using the metal powder of Ti and Al for the...

23 December 2020 7,122 8 View

How to convert Unit/mL enzyme concentration to FPU/mL or FPU/g concentration?

Is there any conversion formula for this purpose.

06 December 2020 3,283 3 View

How many LED bulbs (warm light) will be required for algal growth?

We have 10 glass boxes with 6 inches width and 12 inches depth. How many LED bulbs or halogen lamps will be required to achieve irradiance of 150 umol/s/m2.

23 November 2020 9,414 4 View

What major hardships teachers are going through due to COVID-19?

Teaching Hardships during COVID-19 in 2020 Please highlight what type of hardships, difficulties you as a teacher going through during these trying times due to COVID-19 around the globe? Even...

05 November 2020 4,142 20 View

Anyone can share a test to measure the level of critical thinking skills in university students?

I am PhD scholar, intended to conduct an experimental research to develop critical thinking skills in prospective teachers. if any one developed a test measure please share with me. bests

26 October 2020 4,403 8 View

Please guide me that which theory will relate to the relationships of personality traits, optimism and somatic symptoms disorder?

I have to write the theoretical framework for the course project regarding the relationships of personality traits, optimism and somatic symptoms disorder? Can anybody guide me that which which...

20 October 2020 6,709 4 View

How to measure internal validation of multiple regression using a split-sample method with dichotomous variables?

I would like to validate a regression model using a 50-50 (or 80-20) split method. In my initial multiple regression I have entered all predictor variables using 'enter' technique. My sample size...

20 October 2020 2,849 5 View

Is it dangerous, if samples produce hydrogen gas inside muffle furnace at temperature around 300-400degree celcius?

Geopolymer, derived from flyash, NaOH, Al powder is required a heat treatment inside the muffle furnace. It may cause releasing hydrogen gas inside the furnace. Will it be explosive? or dangerous?...

11 July 2020 3,405 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Malcolm Nobre

Hi Ayesha Seth

Three reactive moieties (amine, sulfhydryl and carbohydrate) in the antibodies are chiefly favored for conjugational purpose. You can refer to the article attached for the protocol.

Article Comparative study of the three different fluorophore antibod...

Best.

Ajeesh Chandrasekharan

As Malcolm described based on the functional groups in the anti-body, you can select the fluorophore. For example, if your antibody has an amine group then you can select fluorophore with succinimidyl ester group and the two would react under basic conditions. Then can purify the product using dialysis/precipitation.