I compared my wild-type protein and a truncation towards binding of RNA by microscale thermophoresis. The truncation shows a V-shape curve resembling biphasic binding. Theoretically the truncation may expose more binding site for RNA. But since I put my Cy tag on RNA (very short oligo, ~10 nt), this becomes technically not possible. Any suggestion as of how I can confirm the biphasic is not artefact?