How to extract nuclei from OCT frozen tissue?

I am trying to extract intact nuclei from OCT-frozen tumor tissue samples. Does anybody have a protocol for doing so? I need as many intact nuclei as possible for performing ATAC-seq on them.

13 September 2020 8,608 1 View

Determination of dc electrical resistivity from FMR lineshape?

Suppose I have FMR line shapes (dP/dH) at different temperatures for a particular frequency, say 10 GHz. If the line shape contains both absorptive and dispersive components, we can fit the line...

27 May 2020 4,140 1 View

How do I edit md_run.mcr file in YASARA Structure for a simulation with respect to pressure, density at 343.15K to check thermostability?

I am aiming a simulation run in YASARA Structure at 343.15K to check thermostability of a mesophile mutant for which i need to edit the md_run.mcr file with respect to Force field pressure control...

27 May 2020 2,725 5 View

How to dissolve a 21-mer synthetic peptide (acidic)?

I'm facing a problem in dissolving two synthetic peptides (21 amino acids long). The peptides have iso-electric points at pH 4.01 and good predicted solubility in water. However, the synthesized...

16 February 2020 6,617 3 View

What is the best mobile phase for separation of caffeine by HPTLC system?

I have used dichloromethane : methanol = 90 : 10 but the caffeine got eluted with the mobile phase. Both the chemicals used were of HPLC grade (Merck).

17 June 2019 6,627 7 View

Hello, does anybody has any 5'-FAM labelled oligos transfection protocol using lipofectamine RNAi MAX for buffalo oocyte?

i need a protocol for transfecting the oocyte with FAM labelled LNA Blockers using Lipofectamine RNAi MAX as a transfecting agent. i use to pool 10 oocytes in 100ul of transfecting solution. kept...

09 July 2017 9,936 1 View

Which buffer is appropriate for mature miRNA quantification HiSpec or Hiflex ?

I am using miScript RT II Kit for cDNA preparation from total RNA pool, this kit provides two buffers hispec and hiflex. on QPCR lower ct values comes with hiflex but not with hiSpec. which one to...

26 February 2017 2,570 2 View

How do I optimize annealing temp of expression primers

Hello, I am using expression primers having restriction sites plus extra 4 base pairs, I have tried gradient PCR with Dream Taq as well as Q5 DNA polymerase but there has been no amplification so...

12 January 2017 2,618 3 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View