I’m a field biologist working on social evolution. I want to switch from micro-satellites to SNPs to determine kinship and sex of individuals (I'm working on a corvid bird). Does anybody know which commercial companies that can do Dartseq for the SNP discovery step and the subsequent SNP typing (e.g. with DD-RAD methods or similar) in Europe or North America? And, what would the first step and the second step cost for 3000 individuals?
Thanks a lot!
As someone that gets given data for similar studies (fingerprinting clonal crops) from lots of different providers I think it's best to just reach out to the companies for quotes.
DArT's new technology DArTag seems to be very popular right now rather than DArTseq.
LGC is offering FlexSeq, CaptureSeq and SNPseq (disappearing soon, but the technology is also offered as Allegro by Tecan if you look up SPET). FlexSeq may actually be the best for you since you will be able to construct haplotypes that then could be more powerful than microsats. LGC also offer KASP assays if you just want SNPs (if you bring this in house and use PACE it can be very cheap). LGC also have European and US centers whereas I'm not sure if DArT have centers exclusively in Australia (I doubt it considering the amount of studies using that platform).
Good luck! Happy to give more details if needed
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