Genescan gives 3 peaks instead of one?

Hi all, I am doing a genescan analysis for a deleted exon in the patient sample. The amplified region is 215 bp. In the patient sample because of homozygous deletion, no peak is observed while in...

23 February 2021 5,645 1 View

How to extract water soluble amino compound from after aqueous work up?

4-Dimethyl amino pyridine is used in reaction.Product formed contains amino group along with amide & is highly water soluble. To remove 4-DMAP aqueous work up done. Please give solution. Thanks.

21 February 2021 1,883 1 View

How doping in nano-particles with same valency affect it's crystal lattice structure?

When doping in case of semiconductor is concerned, we doped Si by trivalent or pentavalent impurity atoms to improve its electronic properties but in case of nano-particles, sometimes we doped...

31 January 2021 7,128 1 View

How to prevent aggregation of nano-particles without using surfactants?

As it is well known fact that nano-particles can easily aggregate during nucleation/ synthesis part due to high surface energy..by reading literature, I came to know that surfactants can help to...

30 January 2021 8,206 6 View

How do I dissociate an antibody used for positive selection of cells in order to re-culture the selected cells?

Hi, I am interested in cells that express a specific receptor. I use a fluorophore-conjugated antibody bound to the receptor followed by MACS positive selection to enrich for this...

21 January 2021 2,245 3 View

Is it possible that insecticides from same group and same mode of action may not show cross resistance?

I could not find any cross resistance between two insecticides from OP group, is this possible?

14 January 2021 9,625 3 View

I'm getting same +ve and -ve value of the blank, which I set for taking fluorescence reading?

I'm checking fluorescence of umbliferon and has set water as blank in two wells. The two wells are showing same reading but one is +ve and another is -ve. Also, the fluorescence can't be negative....

12 January 2021 2,332 3 View

Standard curve for 7 hydroxy coumarin (umbliferon)?

Hi! I am getting a higher fluorescence value for a lower concentration Umblifero solution than compared to a higher concentration solution. I am using the 0.001, 0.0008, 0.0006,0.0004, and 0.0002...

07 January 2021 4,353 5 View

I want to use Gold electrode as a reference electrode for electrodeposition of nanostructures. Can i use it and how ??

How to use a Gold electrode as a reference electrode ??

04 January 2021 4,058 4 View

Basmati rice and GI tag?

You may be aware of the application for Geographical information tag to Basmati rice by India to European Union (EU). Since Basmati rice is traditionally grown, sold, and consumed in Nepal and...

16 December 2020 8,402 3 View

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02 March 2021 3,060 3 View

What do you know about biotechnology art?

What do you know about biotechnology art?

02 March 2021 1,653 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

How was your experience with Biomedical and Biotechnology Journal?

How was your publication experience with BBRJ in terms of peer review and reviewer comments. Did you find it fair and transparent ?

23 February 2021 3,810 6 View

Are the topics below viable research in plant biotechnology?

1. Chemically induced mutagenesis in seed and qPCR detection and amplification of desired trait. 2. Site-directed mutagenesis in qualitative traits of a multiline seed.

04 February 2021 9,069 3 View

Is receiving Invitation to submit a article from the journal is big deal ???

I have recently received a invitation from the journal "Current Pharmaceutical Bio-technology" to submit a research article. As i didn't received any invitation before. so, is it normal to to...

04 February 2021 4,596 23 View

Why is Fermented Plant Juice (FPJ) always diluted with water? Could it be diluted with other solvents such as rice water?

I am currently making a research paper with my classmates in Philippine Science High School and we are trying to find references that mixed Fermented Plant Juice with other solvents instead of...

17 January 2021 5,099 4 View

What steps should one take to address post-harvest disease problems in a supply chain?

If I were to receive an import of a new fruit species in the country and, upon arrival, the whole lot was diseased, what steps do I take to diagnose and solve the problem? Is there a general...

10 January 2021 1,649 7 View

Why negative controls also showing the specific bands of promotor and other regions (GUS, GFP etc.)?

Dear Researchers, I did a transformation of an empty vector (pCAMBIA1301/1302) into my passion fruit plants, for the optimization of the transformation protocol. After transformation; DNA...

06 January 2021 8,465 99 View