Macy Chui :

After Primer-Blast has finished designing primers for your template DNA sequence a graphical interface tool is shown with the different pair of fragments the tool has designed. Simply put your cursor over the amplified fragment you are interested in and Primer-Blast will show you some characteristics of that fragment. Including the PCR product size (that includes forward and reverse primers). In the image you will see what I am describing.

Hi Macy

You could also use the software SerialCloner (http://serialbasics.free.fr/Serial_Cloner-Download.html) for Mac, PC, and linux. This free software is very handy and can do virtual PCR, virtual restriction profiles, plasmids maps.....

The simplest alternative is to blast your primers on the genome that you have been using and note the coordinates. The difference between the minimum and maximum coordinates give the amplification size!

Best

JL

You could upload the sequence of your NAT2 gene and carry out an in silico PCR with your chosen primers and obtain the size of your amplified fragment !

Thank you all I got it!