Luis Rodrigo Arce-Valdés

3 Questions 8 Answers 0 Followers

Questions related from Luis Rodrigo Arce-Valdés

Combinatorial barcoding on single-digest RADseq?

Currently, we are about to begin developing RAD-seq libraries. We are following the single-digest protocol by Amores et al. 2011 (Amores, Angel, et al. "Genome evolution and meiotic maps by...

02 March 2022 3,572 0 View

Which is the best approach for nucleotide substitution model-testing for phylogenetic reconstructions using RADseq data?

I am trying to infer both maximum likelihood and bayesian phylogenetic trees using RADseq reads. I have already created my sets of radtags using Ipyrad which also I used to export the consesus...

25 August 2020 8,186 8 View

Can enough genomic DNA (1ug) be extracted from 3 mg insect samples using Qiagen-DNeasy Blood & Tissue Kit?

We plan to develop RADseq libraries from damselfly larvae grown in the lab, and in this moment our larvae have a size of approximately 5 mm and weight around 3 mg. However, we don't want to keep...

18 December 2019 8,284 0 View