I have already make extract RNA by trizol reagent and using nano-drop to measure the observance at 260-280 nm and RNA purity but the purity of extracted ran is very low the result is below one sample for example
RNA cons. : 194.6 ng/micro litter
260:280 : 1.90
260:230: 0.39
Is the Concentration for RNA good for synthesis of cDNA for clone gene and how to calculate RNA ratio? Why use 500 ng/ml RNA when use prime script rt reagent kit (TakaRa )? Ii can't understand this and if the RNA Concentration is very low what and I do?