The quantum yield (QY) of the quantum dots was measured using quinine sulfate in 0.1 M H2SO4 solution as a reference (QY = 54%). The absolute value of the QY was calculated by taking the standard reference sample with a known QY value using the following formula:
QY=(FArQYr)/(FrA)
where F is the integrated fluorescence intensity of the sample, A is absorbance of the sample and Fr and Ar are the integrated fluorescence intensity and absorbance of the reference, respectively.
The absolute photoluminescence quantum yields (PLQY) of all solid-state compounds were measured using an absolute photoluminescence (PL) quantum yield spectrometer (Quantaurus-QY Plus / FLS1000) equipped with an integrating sphere under ambient conditions.
Thank You so much for your valuable answer Li Jiurong This is the only method. Or can you suggest me another method because the instrument you mentioned above is unavailable here?
I am very sorry, but if one wants to get absolute PLQYs, this method is the only one currently possible. But it is also possible to refer to the solution test method and use a control, as a previous scholar said.@Ashok Kumar