I propose to plot your data on graph paper at large scale and to calculate the area under the curve by cutting and weighing, comparing it to the weight of a piece of the same graph paper of known area.

Thank you very much Peter sir

Dear Savita Rani,

In the software GraphPad Prism you can calculate the area under the curve: https://www.graphpad.com/support/faqid/82/

You can download a 30 days trial version after a free registration: https://www.graphpad.com/demos

Hope it helps.

Regards