I want to know that from SEM images how one can calculate particle size. Here I had attached one SEM image. Can anyone explain me how to calculate particle size?
What we see in the SEM image is grain boundary. The grain can be like platelet, spherical, needle like, etc. In one grain can contain many molecules like alumina and aluminum titanate. That is why sometime the grain in SEM image can be large and very large. If you zoom larger and larger you may find smaller scale but not smaller than 25 nm due to resolution limitations of SEM. SEM image tends to represent grain size not particle size except your SEM resolution close to 25 nm. You can collect TEM image and dynamic light scattering (DLS) to compare to SEM image result. Both TEM and DLS tend to represent particle size.
Hi Neha,
My students use the available software ImageJ (https://imagej.nih.gov/ij/) to calculate grain or particle size from SEM images. After calculating the sizes they plot histograms to exhibit the distribution of the particles size.
Good luck.
Basith
Thank you Dr. Mohammed Abdul Basith Sir for your reply. I'll try this software.
Dear Neha,
As Dr. Mohammed indicate, you can use ImageJ software for calculating particle size of your SEM image. First you have to set scale (i see there is a scale of 200nm in your image). Next you can measure average particles by mean linear intercept method. You can check this video tutorial : https://www.youtube.com/watch?v=JQAZ--c9YfI
Hope this helps. Good luck!
Please see the following video:
https://www.youtube.com/watch?v=wkSrZf3jHV0
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