hello every body i need to calculate the optical density for bacteria agriculturist which the range is optimize and then how to calculate OD when i need mix tow bacteria to study the interaction between them?
thanks a lot
please follow the answers
https://www.researchgate.net/post/How_to_measure_the_concentration_of_bacterial_broth_using_a_spectrophototmeter
Optical density can not be manually estimated. It is an automated reading recorded using spectrometer. It helps us estimate OD by measuring absorbance of a solution at a provided wavelength. For your study, measuring the absorbance at 620 nm will be appropriate. However, we do not have any optimal value of OD for bacterial suspension. You can make a standard fixed dilution of bacterial suspension (possibly of a standard strain of desired bacteria), measure its absorbance at 620 nm and consider it as the reference value throughout your study.
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