Respected sir/ madam

I have done grinding of 100 mg leaf in 3 ml of 0.1% TCA. after centrifugation 1 ml of supernatant + 4 ml of 20% TCA containing 0.5%TBA was waterbathed at 95 0C for 30 min. then O.D was taken at 532 and 600 nm. from this how could i calculate MDA in mM/g FW ?