Dear Sara,

For the calculation of the  lysozyme enzyme activity, you may use the following equation:

Units/ml enzyme = (ΔA450/min Test – ΔA450/min Blank) (df) / 0.001x Volume in mL of enzyme solution

where:

df = dilution factor

0.001 = ΔA450 as per the Unit Definition

To apply this equation to the study described in the file attached to your question, you should substitute ΔA450 with ΔA420 and Volume in mL of enzyme solution should be 0.5. Please note that the incubation time in the study described in the attached communication was 30 minutes. All of your calculations should be based on the results illustrated in Figure 2.

For more information on lysozyme enzyme activity, please see the following link:

http://www.sigmaaldrich.com/technical-documents/protocols/biology/enzymatic-assay-of-lysozyme.html

Hoping this will be helpful,

Rafik

Hi. Thanks a lot Rafik. but what is it factor dilution in my assay? I don't use from any dilution in my sample! I pick supernatant (after centrifugation) and without making any dilution, apply according attached protocol. 

I have dilution process in product standard curve of lycozyme with specific concentration. are this you're purpose about dilution factor or no?

Dear Sara,

The dilution factor in your assay is 1, however, in some other experiments when dilution is done consideration should be given to the dilution factor.

Rafik

thanks a lot for you're reply  dear Rafik. I wish best for you.

Dear Rafik. But I have else question: so when I have to use from standard curve of lysozyme?( my standard curve plotted in micro gram Lysozyme of white egg hen).