Which peak should I consider when calculating ZIF-8's crystalline size from XRD?

Hello, Using the Scherrer's equation, one could calculate, roughly, the crystalline size of ZIF-8. However, I am confused as to which peak in the XRD pattern should be taken for the calculation?...

10 January 2021 8,633 3 View

Could anyone tell me any molecular biology company that sequences mitochondrial genome?

I'm looking for a company to sequence the complete mitochondrial genome of some species of fish.

14 December 2020 3,154 3 View

Why does a Nested PCR protocol amplify samples and not amplify positive control?

I have this problem. Today I used a new positive control of an isolated NC-1 strain in cell culture and the problem persists. I performed a serial dilution test to test the sensitivity of the...

08 December 2020 6,252 2 View

How to avoid the formation of traces and dimers in DNA gel electrophoresis?

07 December 2020 3,052 3 View

Is the pipetting order of the positive and negative controls in the PCR important to avoid in a possible contamination?

I have been watching bands form on my negative controls. I have already changed all reagents, optimized the hygiene of materials and the environment and increased care in pipetting.

07 December 2020 9,090 3 View

How to standardize and obtain a good PCR reaction with samples with a low and variable amount of DNA?

I work with the detection of N. caninum DNA in milk samples in milk samples. The DNA concentrations in my samples range between 2-90 ng / ul. I would like to establish and standardize a final...

01 December 2020 8,792 3 View

Can anyone help me regarding the doubling time of JAWS II cells?

Anyone with experience in growing JAWS II cells could give me an idea of its doubling time? I intend to use these cells for transfection protocols. Any tips to help with growing and transfecting...

01 December 2020 855 3 View

How to know if 87Sr/86Sr is reliable for Sr isotope chemostratigraphy in marbles?

I am currently looking for parameters to know if 87Sr/86Sr data is reliable for chemostratigrahy in marbles, and/or parameters to know if those marbles have had some post-depositional...

30 November 2020 9,032 8 View

Genome sequenced twice in the same technology are very different. How to explain?

Hi dear all, We are studying a fungal disease and we sequenced the same fungi twice, but with different extraction times. One 11 years ago and the another one this year. The strain was extracted...

04 October 2020 271 8 View

At what temperature does graphene oxide start to reduce?

Hello, I am wondering if using temperatures up to 65 C reduce graphene oxide at all. I currently fabricate graphene oxide membranes from a water dispersion (heat up to 40 C during sonication). I...

15 September 2020 6,627 12 View

Is it possible to calculate entalphy of LiBr/H2O or another solution if we know correlation of specific heat without using any software?

Is it possible to calculate entalphy of LiBr/H2O or another solution if we know correlation of specific heat without using any software?

17 January 2021 5,522 3 View

Interested in contributing to a Special Issue in Entropy (MDPI, IF 2.49)?

30 November 2020 1,941 1 View

Which element we can replace in mechanically alloyed FeCoNiAlMn equi-atomic high entropy alloy powder to increase its soft magnetic properties?

Which element we can replace in mechanically alloyed FeCoNiAlMn equi-atomic high entropy alloy powder to increase its soft magnetic properties?

30 November 2020 1,573 5 View

What utility function would be used to reward/punish learning of non local rules defining implicate order?

Given the Meijer paper "A New Premise for Quantum Physics, Consciousness and Fabric of Reality," I am developing an OpEMCSS systems science simulation model. The system is a network of concurrent...

23 November 2020 6,493 1 View

How can we calculate theoretical stacking fault energy of unknown/new complex alloys?

Is there any method/theoretical approach to calculate the stacking fault energy of complex alloys such as high entropy alloys? The theoretical/ approximate value of SFE is needed for alloy design.

16 November 2020 7,074 3 View

What is the relationship between digital information and physical entropy to the aspect of the thermodynamic point of view?

A prime example of the fundamental computational physical interactions, and one that I will emphasize throughout my study, is the tight interplay between logical and thermal considerations at the...

14 October 2020 1,302 4 View

Which is a better criterion for evaluating the performance of a neural network in multi-class classification task (crossentropy loss vs accuracy)?

In terms of evaluating how well a neural network performs in a classification task with the number of classes greater than 2 (for example, classifying an observation into one of the 4 classes),...

11 October 2020 5,152 6 View

Can we have the exergy analysis of a process using fluent software?

For example investigating the entropy generation number or entropy generation through heat and mass flow

10 October 2020 4,540 4 View

Do scalar-tensor-vector gravity theories violate the Bekenstein-Hawking area law to compute the entropy of black holes?

I am looking for the thermodynamics of the cosmological black hole in scalar-tensor-vector gravity theories. I know how should I write and find the thermodynamical quantities and equations for...

26 September 2020 6,383 3 View

Can entropy be reversed? In the Sci-Fi movie TENET, the concept is used to reverse time, is it possible theoretically?

Second Law of Thermodynamics states that as usable energy is lost, chaos increases - and that progression towards disorder can never be reversed.

01 September 2020 2,528 37 View