How to calculate carotenoid content in plant tissue?

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Arvind Singh

Please have a look at these researchgate links and the PDF attachments.

https://www.researchgate.net/publication/260031335_Review_of_methods_for_analysis_of_carotenoids

https://www.researchgate.net/publication/225665478_Spectrophotometric_measurement_of_carotenes_xanthophylls_and_chlorophylls_in_extracts_from_plant_seeds

https://www.researchgate.net/post/Can_we_determine_carotenoid_by_using_UV-spectrophotometer

Best of Luck

Article Review of methods for analysis of carotenoids

Article Spectrophotometric measurement of carotenes, xanthophylls, a...

Hanan Isweiri

http://www.thyssen-web.de/assets/files/fd_documents/sp_buche/uv_vis_pigmente.pdf

Joyeeta Mitra

Concentration of Carotenoids can be calculated using this formula:

Carotenoids = [1000(A470) – 2.27(chl A) – 81.4 (chl B)] / 227

Reference: Szárazová K, Fargašová A. 2007. Phytotoxicity Treatment plant high chromium and nickel. Acta Environmentalica Universitatis Comenianae (Bratislava); 15: 39-52

Kiran Saroy

Thanks all of you :)