Hi Everyone
I am using micro-plate reader to determine peroxidase enzyme (POD) activity in wheat root. I want to do it using molar extinction co-efficient of guaiacol. Another factor to calculate the molar extinction co-efficient in this type of assay is the concentration of hydrogen peroxide. Could you please help me out with your suggestions, how to proceed. please let me know if I need to provide any further information to get your kind help.
Thank you