Its been thrice. i'm gettin' fungal contamination in some of the shoot- culture of Origanum vulgare. I'm constantly monitoring the time of treatment with the bavistin and HgCl2 and yet some culture dies and rest in majority gets contaminated. Plz s'one suggest me tips to get rid of this dilemma. If you have your own protocol for In-vitro multiplication of shoot of O.vulgare
Hi Abhin. I think you should use two surfactants simultaneously like first use mercuric chloride 0.1 % then treat with 1% sodium hypo chloride. If You already have used this, increase the timing of treatment. I am working with pea and i am not getting problems like with You. May this help You...
Treat explant with 0.1% mercuric chloride soln and increase the timing of treatment.
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