Abhin Kumar Megta

3 Questions 12 Answers 0 Followers

Questions related from Abhin Kumar Megta

What should be the pH of Na-phosphate/HEPES buffer for the protein having pI around 9.43(expasy tool)?

Hi, I've been working upon the protein that has pI around 9.43 (expasy tool) and using sodium phosphate buffer (pH- 7.5) in Ni-NTA chromatography. I used to get precipitation with buffer exchange...

04 April 2015 881 4 View

What is an efficient method for the preparation of extract of origanum in methanol and water for checking antibacterial properties?

I've prepared my sample with the aid of ultrasonicator followed by centrifugation at 8000rpm for 10 mins. The sample was not as expected and didn't show any antibacterial effect. Can somebody...

03 March 2014 2,439 8 View

How to avoid Fungal contamination during Invitro shoot multiplication of Origanum vulgare?

Its been thrice. i'm gettin' fungal contamination in some of the shoot- culture of Origanum vulgare. I'm constantly monitoring the time of treatment with the bavistin and HgCl2 and yet some...

03 March 2013 3,629 2 View