I am conducting TNBS assay to determine the number of the primary amine group of a protein. Based on the protocol, I will need to do this assay with my sample and compare with an amino acid. I would like to ask about how to determine the number of amine group per molecule in my protein based on a comparison with an amino acid?
Michael G. Weller
It is assumed that 1 mol of amino acid leads to the same signal as 1 mol of amino groups (mainly epsilon-amino groups of lysine) in your protein. Depending on the reaction conditions, you may get only the number of accessible amino groups.
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