Hello colleagues,
I would like to amply the lignin-degrading gene (i.e., LigX, LigY, LigZ, and LigW) from different types of soil. I have tried to find literature, but unfortunately, there are no articles available where they have quantified those genes from soil samples. There are some cloning/mutagenesis studies available where they amplified those genes and inserted them into E.Coli, but we are yet not successful amply from soil samples. Does anyone have an idea or someone working with ligning-degrading genes quantification? We are interested to quantify those genes from bacteria only.
Thank you and really appreciate your suggestions.
Milan
@ Milan, my assumption is bacteria secrete various oxidative enzymes like DyP-type peroxidases and laccases that may amplify lignin degrading genes.
Please find related articles :
Masai, E., Katayama, Y. and Fukuda, M. 2007. Genetic and biochemical investigations on bacterial catabolic pathways for lignin-derived aromatic compounds. Biosci Biotechnol Biochem. 71: 1–15. Masai, E., Yamamoto, Y., Inoue, T., Takamura, K., Hara, H. and Kasai, D. 2007. Characterization of ligV essential for catabolism of vanillin by Sphingomonas paucimobilis SYK-6. Biosci Biotechnol Biochem. 71: 2487–2492. Hatakka, A. 2001. Biodegradation of lignin. In: Hofrichter M, Steinb_chel A (eds) Biopolymers: biology, chemistry, biotechnology, applications, vol 1. Lignin, humic substances and coal. Wiley VCH, Weinheim, pp 129–180 and....
https://www.frontiersin.org/articles/10.3389/fmicb.2016.01428/full you can read this
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