Hello! I have problems trying to amplify a fragment of 8-10Kb by PCR. I used the phusion Taq from thermos scientific but I didn’t get any results. If someone has experience in this issue, please could you give me some advices?
Arvind Singh
Dear Dora,
Please check these researchgate links for the answer.
https://www.researchgate.net/post/How_do_I_amplify_a_10_kb_fragment_via_PCR
https://www.researchgate.net/post/How_to_amplify_large_fragment_by_PCR
https://www.researchgate.net/post/Does_anyone_perform_long_range_PCR_10kb_with_Platinum_Pfx
https://www.researchgate.net/post/Article_needed
Good Luck
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