When I change the filters of our fluorescent microscope, the sample moves slightly, no matter how carefully I do it.
As a result, images of different channels are not directly mergable. (See the attached picture, where the red and green channels, that are supposed to overlap, differ in at least 10 pixels horizontally)
I am looking for a program or plugin within a program (ImageJ ?) where I could fix this issue and align/move my images prior to merging. I would appreciate your ideas.
There are actually several methods to take care of this, some of which are detailed on the ImageJ forums. Take a look through this and pick your favorite method:
https://forum.image.sc/t/how-to-align-the-image-in-order-to-overlap-the-two-images/8072/2
There are actually several methods to take care of this, some of which are detailed on the ImageJ forums. Take a look through this and pick your favorite method:
https://forum.image.sc/t/how-to-align-the-image-in-order-to-overlap-the-two-images/8072/2
Thanks a lot! I ended up using the ImageJ plugin Align_RGB_planes, and it works perfectly :)
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