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It was assumed that 4E8 T cells should be isolated and activated using plate-bound anti-human CD3, after which would be used for a genome-wide CRISPR screen. (Ref: Genome-wide CRISPR Screens in...
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We have constructed lentivirus transduced U251 cell lines to express (1) EGFRvIII (its amino acid sequence is MRPSGTAGAAFLALLAALCPASRALEEKKGNYVVTDHG..., which is identical to the sequence...
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It is noticed that DNA is toxic to cells in a dose-dependent manner. But I have not grasped the underlying mechanism. I guess there may be some players (proteins or others) involved in this...
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