How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

Which are the best methods to graft the polymeric nano/micro particles with lipids?

Good references (with clearly written methods/protocols) are highly appreciated. Polymers could be any, e.g., PLA, PCL, PLGA, etc. Similarly, lipids could be DSPE, DMPE, DMPA, DPPE, DLPC, DOTAP,...

07 December 2020 5,085 4 View

Which is better for polymer grafting, adding polymer into your nanoparticles or the other way around?

I am having problem with coating my NP (size ~100 nm, irregular shape/cluster, stabilised with citrate). So now I am trying both methods with different results, but I just find it really time...

12 February 2020 2,075 3 View

Which is better for polymer grafting, adding polymer into your nanoparticles or the other way around?

I am having problem with coating my NP (size ~100 nm, irregular shape/cluster, stabilised with citrate). So now I am trying both methods with different results, but I just find it really time...

01 February 2020 9,442 3 View

Use of very long (C>24) pendant chain in grafting polymer ?

I am working on a pour point depressant synthesis project and I already went through a lot of papers but not a single one mentioned using side chain monomer in the grafting process longer than...

19 January 2020 5,036 1 View

Use of very long (C>24) pendant chain in grafting polymer ?

I am working on a pour point depressant synthesis project and I already went through a lot of papers but not a single one mentioned using side chain monomer in the grafting process longer than...

31 December 2019 3,404 1 View

How to efficiently graft two nanoparticles of various sizes?

I am working on grafting two nanoparticles of various sizes, i.e., one is 10-20nm, while the other is around 100nm. Can anyone please tell me a simple, robust, and efficient grafting technique to...

07 August 2019 3,796 5 View

How Cross-link collagen to hydroxyapatite and B-TCP powder?

what's your recommended method for cross linking collagen to hydroxyapatite; and B-tcp powder?

05 June 2019 1,126 4 View

What are the experimental methods to find softness of hairy nanoparticles?

What are the experimental methods to find softness of hairy nanoparticles or specifically, polymer grafted metal nanoparticles (PGNPs) ? I have synthesized polymer grafted metal nanoparticles with...

07 May 2019 4,663 4 View

What are the experimental methods to find softness of hairy nanoparticles?

What are the experimental methods to find softness of hairy nanoparticles or specifically, polymer grafted metal nanoparticles (PGNPs) ? I have synthesized polymer grafted metal nanoparticles with...

04 May 2019 8,600 5 View

Is the weight of grafted polymer much higher than either components?

suppose i take 5 gm of polymer A and graft using 5 gm of polymer B The resultant grafted product would weigh much more than 10 gms Grafting will be done via free radical polymn

18 March 2019 9,279 3 View