I am following a colleague's protocol for isolating a virus from crushed honey bees. The protocol includes pelleting through a 30% sucrose cushion at 103,000g for 6hrs and then a second step of through a CsCl gradient at 115,000g for 24hrs. Both steps are pelleting not banding. The colleague uses 5 ml tubes (13 x 51mm), we will be using 13.2 ml tubes (14 x 89mm). Because our tubes are nearly twice as long (89mm rather than 51mm), can anyone suggest whether the run times need to be longer (and would it be 89/51x as long?).

Many thanks

alan